Figure 2. Prmt5 KO in embryonic myoblasts (Prmt5^MKO) delay postnatal myogenesis and muscle growth.

(A and B) immunofluorescence of Pax7 (A) and MyoG (B) in the left panels and corresponding quantification of cells, in right panels, of WT and Prmt5^MKO TA muscle sections at P7 (n = 3) and P14 (n = 5). Myofiber membranes are stained with dystrophin and nuclei are stained by DAPI sarcolemma. Scale bar, 10 μm.

(C) eMyHC immunofluorescence (left) and quantification of eMyHC⁺ myofibers (right) in TA muscle of WT and Prmt5^MKO mice at P7 (n = 4–5). Scale bar, 10 μm.

(D and E) immunoblotting analysis showing the protein contents of PRMT5, MyoD, eMyHC, and tubulin at P7 (D) and P14 (E) in skeletal muscles of WT and Prmt5^MKO mice. KD, kilodalton size marker.

(F) Body weights of P7 and P14 WT and Prmt5^MKO mice (P7, n = 3; P14, n = 5).

Values are expressed as mean ± SEM. *p < 0.05, **p < 0.01 by t test. See also Figure S3.