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. Author manuscript; available in PMC: 2023 Dec 18.
Published in final edited form as: Cell Rep. 2023 Oct 25;42(11):113329. doi: 10.1016/j.celrep.2023.113329

Figure 5. Prmt5 KO activates autophagy in satellite cells.

Figure 5.

(A) Western blotting showing levels of PRMT5, LC3B, and GAPDH in control (MeOH) and Prmt5 KO (4-OH) myoblasts in the absence or presence of hydroxychloroquine (HCQ), along with quantified levels (normalized to GAPDH) of LC-II/I, as an indicator of autophagy (right). KD, kilodalton size marker.

(B) qPCR analysis of autophagy makers in control (MeOH) and Prmt5 KO (4-OH) myoblasts (n = 4).

(C) Pax7 and LC3B immunofluorescence (left) and the relative intensity of LC3B (right) in Pax7+ cells grown on freshly isolated single myofibers from WT and Prmt5MKO mice (n = 4). Scale bar, 10 μm.

Values are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by t test. See also Figure S5.