Figure 6.

hNSCs in WT and Q175 mice display multiple synaptic inputs

(A) Sample traces of sIPSCs and sEPSCs recorded from hNSCs in a zQ175 mouse in ACSF. (B1 and B2) Summary of the sIPSC and sEPSC frequencies from MSNs and hNSCs categorized by the size of their Na⁺ currents. Statistical significance was measured between groups using Kruskal-Wallis one-way ANOVA on ranks followed by Holm-Sidak pairwise comparisons; ∗p = 0.024 for zQ175 Na⁺ > 1,000 pA versus WT Na⁺ < 1,000 pA. (C) Responses evoked by electrical stimulation (0.1–0.5 mA, 1-ms duration) of host striatal neurons (about 200 μm lateral to the graft) in a hNSC from a zQ175 mouse. Glutamatergic (V_h = −70 mV) and GABAergic (V_h = +10 mV) responses were reliably evoked by electrical stimulation. Responses were blocked by glutamate and GABA_A receptor antagonists respectively. (D) Cell membrane properties recorded at a holding potential of −70 mV. (E) Fluorescent image of a recorded and biocytin-filled MSN (yellow arrow pointing to filled neuron [red]) near SC121-immunostained cells and processes (green). (F1 and F2) Effects on inhibitory (left) and excitatory (right) synaptic activity. Statistical significance was measured between groups using Kruskal-Wallis one-way ANOVA on ranks followed by Holm-Sidak pairwise comparisons, In (F), ∗∗p < 0.001 for WT versus zQ175-Veh and ∗p = 0.04 for zQ175-Veh versus zQ175-hNSC. In (G), ∗p = 0.047 for WT versus zQ175-Veh.