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. 2023 Oct 10;114(12):4747–4762. doi: 10.1111/cas.15989

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© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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HK2 elevation rescues the impact of DLGAP1‐AS2 knockdown on osteosarcoma (OS) cellular behaviors. (A) Transfection efficacy of pcDNA3.1/HK2 detected by RT‐qPCR. (B) EdU assay of the proliferative abilities of U2OS and Soas2 cells transfected with sh‐NC, sh‐DLGAP1‐AS2, and sh‐DLGAP1‐AS2 + HK2. (C) The apoptotic behaviors of OS cell lines transfected with the indicated plasmids were evaluated by TUNEL assay. (D) Wound healing assay of cell migration in indicated groups. (E) Transwell assay of cell invasion in indicated groups. (F) Western blotting of the protein levels of EMT‐related markers in indicated groups. **p < 0.01, ***p < 0.001.