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. 2023 Oct 10;114(12):4747–4762. doi: 10.1111/cas.15989

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© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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DLGAP1‐AS2 knockdown inhibits osteosarcoma (OS) progression via the miR‐451a/HK2 axis‐mediated glycolysis. (A) Western blotting of HK2 protein level in control, sh‐DLGAP1‐AS2, and sh‐DLGAP1‐AS2 + miR‐451a inhibitor groups. (B) EdU assay of the proliferative abilities of U2OS and Soas2 cells. (C) extracellular acidification rate (ECAR) and (D) OCR of U2OS and Soas2 cells in control, sh‐DLGAP1‐AS2, and sh‐DLGAP1‐AS2 + miR‐451a inhibitor groups. (E) Lactate production and (F) ATP level in experimental groups. **p < 0.01.