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. 2005 Mar 30;33(6):1825–1833. doi: 10.1093/nar/gki329

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Short oligonucleotides bind to mRNA. mRNA from pJD366-18 T7 run offs was incubated with the joining oligonucleotide J1 and subjected to nuclease treatment. Lanes 1–4 contain mRNA without the oligonucleotide and lanes 5–8 mRNA with oligonucleotide. Lanes 2 and 6 were treated with Mung Bean nuclease; lanes 3 and 7 with RNaseH; lanes 4 and 8 with RNaseT1; and lanes 1 and 5 untreated. The arrow indicates the 5′ 110 nt fragment after incubation with J1 and RNaseH (lane 7).