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. 2023 Sep 19;114(12):4664–4676. doi: 10.1111/cas.15966

FIGURE 2.

FIGURE 2

MK1775 suppresses trifluridine (FTD)‐induced DNA damage responses (DDRs) by cyclin‐dependent kinase 1 (CDK1) activation. (A) Gene dependency correlation among DNA damage repair pathway genes and CDK1. Matrix of Pearson r values calculated from all cell lines included in the data set (712 cell lines). Data set: DEMETER2_v6 combined RNAi (DepMap: https://depmap.org/portal/, Broad Institute). (B, C) Immunoblots of DDR checkpoints, which were suppressed after exposing the cells to a Chk1 inhibitor (LY2606368) and WEE1 inhibitor (MK1775) with increasing concentrations. (D) With FTD, MK1775 not only activated CDK1 but also induced DNA double‐strand breaks, indicated by g‐H2AX. (E) Flow cytometry showing the percentage of mitotic cells, which is indicated by p‐hH3 following the treatment of FTD, MK1775, and a combination of them.