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. 2005 Mar 30;33(6):1859–1873. doi: 10.1093/nar/gki332

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Overexpression of CREB-H inhibits S-phase entry. (A) pcDNA3.1/V5-β-gal, pcDNA3.1-CREB-H and pcDNA3.1-CREB-HΔTC were transfected into HepG2 cells for 16 H. Cells were then treated with 10 μM BrdU for 6 h, and fixed. Cells were subsequently stained with α-BrdU-fluorescein and α-V5 or α-CH8 antibodies. Red (BrdU) and green (β-gal, CREB-H or CREB-HΔTC) fluorescent signals were overlaid in panels 3, 6 and 9. The same fields are shown in panels 1–3, 4–6 and 7–9. The arrows indicate transfected cells. Similar results were obtained from three transfections.