Figure 5.

Detection of increased GFP fluorescence by flow cytometry analysis after RNAi-mediated knockdown of NMD factors. (A) hUpf1 or hSmg6 was knocked down in the NMD reporter gene-expressing HeLa cells as in Figure 4, and the cells were analysed by flow cytometry 4 days post-transfection. (B) From an aliquot of the same cells used for flow cytometry, relative reporter mRNA levels were determined by real-time RT–PCR. Average numbers of four PCR runs, normalized to endogenous GAPDH mRNA levels, are shown.