Table 1.

Sequence errors in input and consensus shuffled DNA

Mutation	Deletion	Insertion	G/C to A/T	A/T to G/C	G/C to C/G	G/C to T/A	A/T to C/G	A/T to T/A
Mismatches	—	—	G:T/A:C	A:C/G:T	G:G/C:C	G:A/T:C	G:A/T:C	A:A/T:T
Input DNA	18	1	15	1	3	4	1	0
Consensus Shuffling	3	0	2	0	2	5	0	0

Deletion, insertion, transition and transversion mutations were quantified after sequencing DNA from randomly selected colonies for the input populations and the populations after two rounds of consensus shuffling. The reported mutations are for the combined input populations (20.8 and 16.3 kb) and consensus shuffled populations (20.0 and 21.5 kb) reported in Figure 3. The two types of mismatched base pairs that arise after re-hybridization of the gene synthesis product are indicated below each transition or transversion mutation.