Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 18;14:8399. doi: 10.1038/s41467-023-44197-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Binding of MoSPAB1^ΔSP to the Bsr-d1 promoter in yeast one-hybrid assay. b Activation of the Bsr-d1 promoter by MoSPAB1 in luciferase assay using N. benthamiana. Luciferase signals were imaged (left panel) and measured (right panel) using a dual-LUC assay 48 hr after infiltration. The positive control contains transcription factor EAT1 and OsLTPL94 promoter driving reporter. EV contains 35 S:YFP as the negative control. firefly luciferase (LUC) activities were normalized to Renilla luciferase (REN). Values are mean ± SD, n = 3 biologically independent samples. Data analyzed by one-way ANOVA followed by two-sided least significant difference (LSD) test for multiple comparisons. Different letters indicate significant differences (P < 0.05). P-values are shown in the Source Data file. c Luciferase activity assay for MoSPAB1^ΔSP binding to the Bsr-d1 promoter in rice protoplasts. The fluorescence signals of LUC and REN (internal control) were detected after rice protoplasts were co-transfected with the vectors for 16 h. Values are mean ± SD, n = 3 biologically independent samples (Student’s two-sided t-test, ***P < 0.001). d DNA affinity purification (DAP)-qPCR analysis for enrichment of Bsr-d1 promoter DNA by recombinant His-MoSPAB1^ΔSP protein. The ratio of His-MoSPAB1^ΔSP/6×His ≥ 2 was used as the threshold for significance. Values are mean ± SD, n = 3 biologically independent samples (Student’s two-sided t-test, *P < 0.05, n.s. indicates no significance). e A conserved sequence bound by MoSPAB1^ΔSP was identified using the MEME suite version 5.4.1. f Binding of His-MoSPAB1^ΔSP to probe 1 of the Bsr-d1 promoter. Similar results are obtained from three independent biological experiments. g Binding of His-MoSPAB1^ΔSP to probe 2 of the Bsr-d1 promoter. Similar results are obtained from three independent biological experiments. Source data are provided as a Source Data file.