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. 2023 Dec 18;14:8399. doi: 10.1038/s41467-023-44197-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Detection of MoSPAB1 domains required for binding to the conserved cis-element using EMSA. The regions of amino acids 27-47, 103-123, 137-191, and 207-271 of MoSPAB1^ΔSP were deleted one by one, tagged with 6×His. Similar results are obtained from two independent biological experiments. b A LOGO sequence of the critical peptide (aa 103-123) of MoSPAB1. The conserved motif was predicted by aligning 45 homologous proteins using the MEME Suite. c A 3-D structure model for MoSPAB1 constructed by I-TASSER. The red portion represents the critical aa 103-123 region. Completely conserved amino acids are labeled. C-score = -1.61. Estimated TM-score = 0.52 ± 0.15. Estimated RMSD = 9.8 ± 4.6 Å. d Interaction of CsSPAB1^ΔSP from Colletotrichum sublineola with the SbBsr-d1 promoter in luciferase activity assay using N. benthamiana leaves. Values are mean ± SD, n = 3 biologically independent samples. e Interaction of CfSPAB1^ΔSP from Colletotrichum fructicola with the AcBsr-d1 promoter in luciferase assay using N. benthamiana leaves. f Transient expression of CsSPAB1^ΔSP in N. benthamiana increases host susceptibility to C. sublineola. The pre-wounded Agrobacterium-infiltrated leaves were detached and inoculated with C. sublineola mycelial plugs after 24 hr post-infiltration. Lesion areas were denoted by circles, and scored 8 days post inoculation. Values are mean ± SD, n = 7 biologically independent samples. g Transient expression of CfSPAB1^ΔSP in N. benthamiana increases host susceptibility to C. fructicola. Values are mean ± SD, n = 4 biologically independent samples. In (d–g) Data are analyzed by two-sided t-test, ***P < 0.001, **P < 0.01, *P < 0.05. Source data are provided as a Source Data file.