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. 2023 Dec 18;13(1):188. doi: 10.1038/s41408-023-00962-z

Fig. 3. Tasquinimod inhibits AML cell proliferation and reduces colony formation by targeting of mTOR-ER stress signaling in vitro.

Fig. 3

A Cell viability of tasquinimod-treated KG-1a and MOLM-13 cell lines (10 and 25 µM) was analyzed using a CellTiter-Glo assay after 24 and 48 h (n = 4). B Cell proliferation of tasquinimod-treated AML cells (10, 25 μM) was investigated using BrdU staining after 24 and 48 h (n = 4). C Methylcellulose colony formation assays were performed for KG-1a and MOLM-13 cell lines treated with vehicle or tasquinimod (10, 25 μM) for 14 days. D Quantification of the colony numbers is shown (n = 4). E–G KG-1a and MOLM-13 cells were cultured with tasquinimod at indicated concentrations (10, 25 μM) for 48 h. Whole-cell lysates were subjected to Western blot analysis using anti-p-mTOR, mTOR, p-P70S6K, P70S6K, p-S6K, S6K, p-4E-BP1, 4E-BP1, GRP78, p-eIF2a, eIF2a, ATF4, p21, p27, S100A9, puromycin, SLC7A11, SLC7A5 and anti-β-Actin antibodies (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001, One-way ANOVA, Error bars indicate SD.