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. 2023 Dec 18;13(1):188. doi: 10.1038/s41408-023-00962-z

Fig. 5. Combination of S100A9-siRNA with venetoclax has additive effects on AML cell apoptosis through downregulation of c-MYC and BCL2.

Fig. 5

S100A9-siRNA combined with venetoclax induced apoptosis of KG-1a and MOLM-13 cells. A KG-1a and MOLM-13 cells were pre-plated in RPMI medium with 10% fetal bovine serum in the presence or absence of siS100A9 (20 nM) for 48 h and add venetoclax (250 nM) co-culture for 24 h. Annexin-V/7-AAD based flow cytometric assay for determination of apoptotic cells. B Venn’s diagram of protein expression with different intensity between venetoclax vs combo and siRNA vs combo in MOLM-13. C The bubble plot shows the top 20 differentially regulated (activated/suppressed) pathways in combo group compared with siRNA (72 h) (n = 3). D, E KG-1a, MOLM-13 cells were cultured with tasquinimod at indicated concentrations (10, 25 μM) for 48 h. Whole-cell lysates were subjected to Western blot using anti-p-mTOR, mTOR, p-P70S6K, P70S6K, p-S6K, S6K, p-4E-BP1, 4EBP1, BCL2, c-MYC and anti-β-Actin antibodies (n ≥ 3). F Graphical abstract of siS100A9 and tasquinimod mediated effects, created with Biorender.com. *p < 0.05, **p < 0.01, ***p < 0.001, One-way ANOVA, Error bars indicate SD.