Table 1.
Sequencing results of synthetic cat genes with or without EMC treatment of the primary ligation product
| Clone #: number of errors (xi) | Sequence length (li) | Endonuclease treatment | Nature of the errors |
|---|---|---|---|
| ss#1: 4 | 616 | 0 | A>G, C>T, C>T, C>T |
| ss#2: 5 | 616 | 0 | Del C, T>C, C>T, C>T, G>A |
| ss#3: 7 | 616 | 0 | Del TA, Del C, T>C, Del A, T>C, C>T, Del T |
| ss#4: 6 | 616 | 0 | Del CC, C>T, T>G, C>T, C>T, T>C |
| ss#5: 3 | 616 | 0 | Del T, G>T, A>G |
| ss#6: 4 | 616 | 0 | G>T, C>T, G>A, Ins C |
| ss#7: 4 | 531 | 0 | C>T, T>C, G>A, C>T |
| ss#8: 3 | 616 | 0 | Del A, Del C, C>T |
| ss#9: 6 | 616 | 0 | G>A, G>A, Del T, C>T, A>T, G>T |
| ss#10: 2 | 616 | 0 | Ins C, Del G |
| ds#1: 3 | 616 | 0 | Del A, Del G, A>T |
| ds#2: 5 | 616 | 0 | Ins G, C>G, Del T, C>T, C>G |
| ds#3: 2 | 616 | 0 | Ins C, G>A |
| ds#4: 3 | 616 | 0 | Ins C, A>T, C>T |
| ds#5: 3 | 616 | 0 | Ins C, G>T, C>G |
| ds#6: 4 | 616 | 0 | G>T, C>T, T>A, C>T |
| ds#7: 3 | 398 | 0 | C>T, Del CC, A>G |
| ds#8: 3 | 616 | 0 | G>A, C>T, C>T |
| ds#9: 5 | 616 | 0 | Del G, Del C, C>T, C>T, T>C |
| ds#10: 3 | 571 | 0 | C>T, T>A, Ins C |
| e7#1: 0 | 616 | 24 h endo VII | |
| e7#2: 0 | 616 | 24 h endo VII | |
| e7#3: 3 | 616 | 24 h endo VII | G>T, C>T, G>A |
| e7#4: 2 | 616 | 24 h endo VII | C>T, A>T |
| e7#5: 0 | 616 | 24 h endo VII | |
| e7#6: 1 | 616 | 24 h endo VII | T>A |
| e7#7: 1 | 616 | 24 h endo VII | C>T |
| e7#8: 2 | 616 | 24 h endo VII | C>T, G>A |
| e7#9: 0 | 159 | 24 h endo VII | |
| e7#10: 1 | 616 | 24 h endo VII | C>T |
| e5#1: 0 | 616 | 4 h endo V | |
| e5#2: 3 | 616 | 4 h endo V | Del C, G>T, C>T |
| e5#3: 0 | 616 | 4 h endo V | |
| e5#4: 1 | 616 | 4 h endo V | T>A |
| e5#5: 1 | 613 | 4 h endo V | A>G |
| e5#6: 3 | 616 | 4 h endo V | G>A, C>G, C>T |
| e5#7: 1 | 513 | 4 h endo V | G>T |
| e5#8: 0 | 526 | 4 h endo V | |
| e5#9: 2 | 616 | 4 h endo V | C>T, A>T |
| e5#10: 1 | 613 | 4 h endo V | C>T |
Error notation: single nucleotide changes are shown as ‘correct base’ > (was changed to) ‘detected base’, e.g. A>G means instead of an expected A, a wrong G was found. Insertions of a nucleotide are indicated as ‘Ins’ followed by the nucleotide that are found additionally, e.g. Ins C means the detection of an additional C. Deletions of nucleotides are indicated as ‘Del’ followed by the missing nucleotide(s), e.g. Del CC means that two consecutive Cs are missing. All endonuclease reactions contained no additional exonuclease. Post-EMC amplifications were done with Vent DNA polymerase.