Fig. 2. Lys24 acetylation of NLRP3 promotes inflammasome activation.

a–c BMDMs from Nlrp3^WT/WT (n = 3) or Nlrp3^K24/K24R (n = 3) mice were treated with LPS (100 ng/mL, 3 h) with ATP (5 mM, 1 h), nigericin (10 μM, 1 h), MSU (200 μg/mL, 6 h), Flagellin transfection (2 μg/mL, 1 h) or poly (dA:dT) transfection (1 μg/mL, 16 h). a, b ELISA analysis of IL-1β and TNF-α in supernatants. c Release of LDH in supernatants. d–h Nlrp3^WT/WT or Nlrp3^K24/K24R male mice of 6–8 weeks were injected with Saline (n = 3 biologically independent mice) or LPS (20 mg/kg, i.p.) (n = 7 biologically independent mice) for 12 h. d, e ELISA analysis of IL-1β and TNF-α in serum. f representative H&E images lung sections. Scale bar = 100 μm. g lung injury score. h Immunoblot analysis of IL-1β and caspase-1 from lung of Nlrp3^WT/WT or Nlrp3^K24/K24R mice. casp-1(caspase-1). Results are represented as mean ± SD. Statistical analyses were carried out via two-way ANOVA with the Bonferroni test for (a–e, g). Source data are provided as a Source Data file.