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. 2023 Dec 18;14:8396. doi: 10.1038/s41467-023-44203-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Co-IP analysis of interaction between the endogenous NLRP3 or KAT5 in peritoneal macrophages treated with LPS (100 ng/mL, 3 h) along or with nigericin (10 μM, 1 h). b GST pull-down analysis of GST-KAT5 and His-NLRP3. c Immunoblot analysis of acetylation level of NLRP3 from Kat5^WT/WT or Kat5^S86A/S86A peritoneal macrophage treated with LPS (100 ng/mL,3 h) along or with nigericin (10 μM,1 h). d Immunoblot analysis of acetylation level of NLRP3 from peritoneal macrophage transfected with Ctrl or KAT5 siRNA treated with LPS (100 ng/mL, 3 h) along or with nigericin (10 μM, 1 h). e Immunoblot analysis of acetylation level of NLRP3 in vitro. The recombinant His-NLRP3 was incubated with GST-KAT5 in the presence or absence of acetyl-CoA (Ac-CoA). Typical photographs are representative of three biological independent experiments with similar results. Source data are provided as a Source Data file.