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. 2023 Dec 18;14:8405. doi: 10.1038/s41467-023-44098-x

Fig. 7. Metabolic dysfunction and reduced ERR activity accompany progressive age-related renal decline in vivo.

Fig. 7

a Relative ERR protein abundance in 7 vs 28 day MpT tissue. b Analysis and quantification of secretion rate in 7, 28 and 35 day MpTs. Analysis and quantification of 2-NBDG uptake (c–e), BODIPY C12 (f–h), LipidTox density (i–k), peroxisome (CapaR>SKL-GFP) density (l–n), ThiolTracker (o–q), MitoSox (r–t), ATP red (u–w) and ATF4 activity (4E-BP-dsRed, reporter of the Integrated Stress Response) (x–z). Analysis and quantification of ATP red staining in CapaR>G6PD-RNAi tubules (aa–ac). Summary schematic of metabolic changes associated with aging (ad). Data represented as box and whisker plots (lower and upper hinges correspond to the first and third quartiles, median line within the box, whiskers extend from the hinge to the largest/smallest value, at most 1.5* interquartile range of the hinge) with all data from MpT main segment sections (e, h, k, n, q, t, w, z, ac), or secretion of individual kidneys (b) shown as overlaid points. NS Not Significant, *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired two-tailed t tests or ANOVA followed by Tukey multiple comparisons test where appropriate). p values: (b) 7 vs 28 day p = 0.0232, 7 vs 35 day p = 0.000168, (e) p = 0.00759, (h) p = 0.0282, (k) p = 0.00258, (n) p = 0.0101, (q) p = 0.000123, (t) p = 0.00369, (w) 7 vs 35 day p = 0.0148, (z) p < 0.0001, (ac) p = 0.000149. p values where p > 0.05 labelled as NS. For analysis of fluorescent reporters/dyes, two images of different sections of the MpT main segment per fly were imaged. All images representative of >5 tubules. All images are maximum z projections. b n = 29 7 day, 22 28 day and 13 35 day tubules, (e) n = 11/12 tubules per condition, (h) n = 12 tubules per condition, (k) n = 12/13 tubules per condition, (n) n = 6 tubules per condition, (q) n = 6 tubules per condition, (t) n = 11 tubules per condition, (w) n = 7 tubules per condition, (z) n = 6 tubules per condition, (ac) n = 11 tubules per condition. Source data are provided as a Source Data file.