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. 2023 Dec 19;14:8423. doi: 10.1038/s41467-023-44152-8

Fig. 6. OX40 agonism alters CD4 T cell phenotype and retention at late timepoints.

Fig. 6

a–g Wildtype C57BL/6 mice at four weeks post-infection treated with 100 μg α-OX40 agonist or isotype control antibody injections twice weekly for two weeks. Mice harvested at 0, 14, and 14 days post-antibody treatment for flow cytometry or lung bacterial load. b Lung bacterial load of α-OX40 or isotype control antibody treated mice. P values calculated with an unpaired two tailed t-test, adjusted for multiple comparisons. Mice per group: 4 for day 0, 5 each for Isotype or OX40 at day 14, and 5 each for Isotype or OX40 at day 42. c Number of CD4 T cells in the lungs of α-OX40 or isotype control antibody treated mice. Gated on live CD4+ CD44+ cells. Cell numbers assessed using counting beads. P values calculated with an unpaired two-tailed t-test, adjusted for multiple comparisons, 5 mice per group. d, e Frequency of parenchymal CD4 T cells in the lungs of α-OX40 or isotype control antibody-treated mice. CD45.2 fluorescent antibody injected immediately before harvest identifies vascular cells. Gated on live CD4+ CD44+ IV- cells. P values calculated with an unpaired two-tailed t-test, adjusted for multiple comparisons. Mice per group: 6 for day 0, 5 each for Isotype or OX40 at day 14, and 5 each for Isotype or OX40 at day 42. f, g Frequency of terminally differentiated CD4 T cells in the lungs of α-OX40 or isotype control antibody-treated mice. Gated on live CD4+ CD44+ CX3CR1+ KLRG1+ cells. P values calculated with an unpaired two-tailed t-test, adjusted for multiple comparisons. Mice per group: 3 for day 0, 5 each for Isotype or OX40 at day 14, and 5 each for Isotype or OX40 at day 42. Error bars indicate standard deviation.