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Intracellular citrate levels in wild-type and mutant cells
| Strain (relevant genotype) | Citric acid concn (μg/mg of protein)a
|
|
|---|---|---|
| T0 | T2 | |
| JH642 (wild type) | 0.45; 0.12 | 0.34; 0.17 |
| MAB160 (citB) | 1.06; 0.83 | 2.14; 2.26 |
| JCB61 (citZAB) | 0.46; 0.23 | 0.15; 0.13 |
The concentration of citric acid (calculated as the anhydrous acid) in cells harvested at the end of (T0) and 2 h after (T2) exponential growth was measured as described in Materials and Methods. Data from two independent assays are shown.
