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. 2023 Dec 18;9:166. doi: 10.1038/s41531-023-00616-8

Fig. 6. DNA oxidative damage and signs of senescence in GBA-PD organoids.

Fig. 6

a Extracellular concentration of 8-OHdG in DIV30 organoids measured by ELISA. The data represent a summary of four independent differentiation experiments for all cell lines. Wilcoxon T-test; ***p < 0.001. b High content automated image analysis showed decreased MFI of LAMINB1 (green) within Sox+ population (red). Data is a summary of eight independent differentiation experiments, normalized to the average of controls per organoid batch. Wilcoxon T-test; **p < 0.01 (scale bar, 20 μm). c, d Representative images of HP1γ (green) and SOX2 (red) in CTRL2 and PD2 organoid sections acquired at 60× (C) (scale bar, 10 μm). Quantification of MFI of HP1γ within the SOX2+ population (D left) and proportion of neural precursor cells (SOX2+) expressing high levels of HP1γ (D right) in DIV30 organoid sections acquired at 20× using the automated image analysis pipeline. Data represents a summary of five independent differentiation experiments normalized to the mean of the controls per batch. Wilcoxon T-test; *p < 0.05, **p < 0.01. e Senescence-associated β-galactosidase staining (blue) of DIV30 organoids (scale bar, 200 μm, 4×; 100 μm, 10×).