Figure 3.

FAK2 inhibition attenuates injured muscle tissue cellular infiltration and osteogenic connective tissue progenitor colony-forming cell activity (CTP-O). Following blast-related polytraumatic extremity injury and 6 days of vehicle or FAK2 inhibitor treatment (PF573228, 10 mg/kg/day, 6.94 µg/kg/min), muscle tissue surrounding the amputation site was harvested on POD-7. Viable nucleated cells were isolated using collagenase digestion, counted and plated in osteogenic culture media. (A) Number of nucleated cells isolated per gram of muscle. (B) Number of assayable CTP-Os per 1 x 10⁶ plated cells. (C) Quantification of number of CTP-Os per gram of tissue by crystal violet staining after 10 days of in vitro culture in osteogenic media. Visible macroscopic colonies containing > 50 cells were enumerated. Data represents mean values ± SEM. * indicates p <0.05.