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. Author manuscript; available in PMC: 2024 Jan 28.
Published in final edited form as: J Am Chem Soc. 2023 Jun 15;145(25):14019–14030. doi: 10.1021/jacs.3c03887

Figure 4. Conformation of CoA-αSFL in complex with hNatB by smFRET.

Figure 4.

Representative single molecule FRET histograms fit to Gaussian distributions. Mean FRET efficiencies (ETeff) are indicated for CoA-αSFL alone (black dashed lines) and in complex with hNatB (red dashed lines). The histograms show no change in ETeff between residues 9 and 72 upon complex formation with hNatB; change in ETeff consistent with expansion between positions 9 and 94 as well as between positions 9 and 136. αS(19-n) refers to measurements made with CoA-αS-C48819π594n (13a, 13b, or 13c). The complete set of histograms and details of data fitting are given in the SI.