FIG. 3.

Constitutive expression of the SOS response results in rapid repair of CPDs and 6-4PPs and eliminates rifampin inhibition of genomic CPD repair. Monoclonal antibodies specific for CPDs (A) and 6-4PPs (B) were used in an immunoassay with DNA isolated from DM1187 lexA51(Def) cells at the indicated times after UV irradiation with 40 J/m². □, untreated cells; ◊, cells treated with 50 μg of rifampin per ml. Points represent the average repair calculated from at least two immunoslot blots of samples from each of two independent biological experiments. Each error bar represents 1 standard deviation calculated from the averages of two independent experiments. Error bars not shown are obscured by the datum points.