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. Author manuscript; available in PMC: 2023 Dec 19.
Published in final edited form as: Cell Rep. 2023 Oct 3;42(10):113188. doi: 10.1016/j.celrep.2023.113188

Figure 1. MC3R is required for the activation of AgRP neurons by fasting.

Figure 1.

(A‒D) 2- (A and C) and 24-h (B and D) food intake of WT and MC3R KO male and female mice following a 24-h fast (n = 8–10 mice for all groups).

(E‒J) qPCR analysis of hypothalamic AGRP, NPY, and POMC mRNA levels in WT and MC3R KO male and female mice over a 48-h-fasting time course (n = 3–9 mice for all groups).

(K‒M) Representative images of GFP and cFos immunostaining, and quantifications of the percentage of cFos-positive GFP cells and the number of GFP cells in the ARC of 24-h-fasted WT and MC3R KO NPY-GFP male mice.

Scale bar, 100 μm (n = 4 mice for all groups). Data are plotted as mean, and all error bars represent the SEM. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 in unpaired Student’s t test and two-way ANOVA with Sidak’s post hoc test.