FIG. 1.

Physical map of the genes coding for AMO, the DNA cassettes used, and Southern hybridization to a probe for amoA and for the kan cassette. (a) The three genes coding for known and putative AMO peptides and the probe used to detect amoA are shown. The BamHI (B) restriction site created for the insertion of the cassettes is shown. The sizes of the EcoRI (E) fragments for the two gene copies are approximately 5 and 6.5 kb. (b) The lacZ/kan and kan cassettes that were inserted into the BamHI site. (c) Southern hybridization to the amo probe and the kan probe. wt, N. europaea wild-type strain.