FIG. 3.

AMO mRNA levels during growth of the wild-type, A141, and A142 strains. The optical densities and the time elapsed at the sampling time are shown. The position for the full-sized AMO mRNA (about 3.5 kb) is indicated by an arrow. The hybridization to the rRNA after stripping the same blot is shown below the amo panel. The amo levels are given as a percent of the wild-type level for each optical density group. These levels were calculated by dividing the intensity observed in the amo signal by the intensity observed in the rRNA signal. The relative intensity for each time point was divided by the wild-type intensity and this value was multiplied by 100. The truncated amo transcript can be observed as an extra band in the amo hybridization and corresponds to approximately 2.1 kb. WT, wild type.