TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli | ||
| DH5α | F′ φ80dlacZΔM15 endA1 recA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | 9 |
| InvαF′ | F′ endA1 recA1 hsdR17(rK− mK+) supE44 thi-1 gyrA96 relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 | Invitrogen |
| N. europaea | ||
| ATCC 19178 | American Type Culture Collection | |
| A141 | Insertion of lacZ/kan into the BamHI site of amoA1 | This study |
| A142 | Insertion of lacZ/kan into the BamHI site of amoA2 | This study |
| A1 | Insertion of kan into the BamHI site of amoA1 | This study |
| A2 | Insertion of kan into the BamHI site of amoA2 | This study |
| Plasmids | ||
| pNHA10 | 842-bp PCR fragment containing most of amoA cloned into pCRII vector | This study |
| pNHA11 | pNHA10 with a mutation in amoA at position 715 (24), creating a BamHI site | This study |
| pNHA12 | amoA insert cut out of pNHA11 with EcoRI and cloned into EcoRI site of pRL139 | This study |
| pNHA14 | lacZ/kan fragment from pKOK6.1 inserted into BamHI site of pNHA12 | This study |
| pNHA15 | kan from pUC4 pKSAC inserted into BamHI site of pNHA12 | This study |
| pUC4 KSAC | Plasmid containing the kan cassette from Tn903 | Pharmacia |
| pKOK6.1 | Plasmid containing a promoterless lacZ and kan with its own promoter | 20 |
| pCRII | TA PCR cloning vector (Kanr AmprlacZa f1ori ColE1 ori) | Invitrogen |
| pRL139 | pUC19 with a symmetrical multiple cloning site | 5 |