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. 1998 Jul;180(13):3360–3367. doi: 10.1128/jb.180.13.3360-3367.1998

FIG. 6.

FIG. 6

Western transfer analysis of total-cell proteins from various Bacillus strains probed with polyclonal B. subtilis 168 RTP antibodies as determined by the ECL-Plus detection system (see Materials and Methods). Only the portion of the membrane relevant to RTP is shown. The position of RTP (5 ng, 14.5 kDa) in lanes 1 and 8 is indicated. Lanes: 2, B. subtilis 168; 3, SU187 (6); 4, B. amyloliquefaciens; 5, B. atrophaeus; 6, B. licheniformis 5A2; 7, B. licheniformis FD01. The lower band in lanes 2 to 7 and the upper band in lanes 6 and 7 represent cross-reacting species. An equal amount of cell protein from each strain as estimated from Coomassie blue staining was loaded into lanes 2 to 7.