FIG. 2.

Reverse-phase chromatography of hydrolysates of gp260 with Achromobacter protease I. After gp260 and Achromobacter protease I (200:1 by molar ratio) were incubated at 37°C for 15 h, the hydrolysate was fractionated with a μBondasphere C₁₈ reverse-phase chromatography column with a linear gradient of 5 to 40% acetonitrile in 0.1% trifluoroacetic acid. The numbered peaks were collected, and their amino acid sequences were determined.