FIG. 3.

Identification of gp260 as Sed1p. (A) Comparison of N-terminal and internal amino acid sequences of gp260 and Sed1p. The amino acid sequences of the numbered peaks in Fig. 2 were determined with a protein sequencer. X, unidentified amino acid; ∗, sequence identified after the N terminus of gp260 was deblocked with pyroglutamate aminopeptidase. Numbers indicate the residue numbers of Sed1p (6). (B) Amino acid sequence of Sed1p obtained from the literature (6). Underlines indicate sequences that were obtained from sequencing gp260 and its fragments. Sequences with double underlines are terminal hydrophobic sequences. Putative N-glycosylation sites are designated as shadowed letters. Cysteine residues are shaded.