FIG. 5.

Analysis of cell wall proteins prepared from wild-type and sed1 disruptant cells. The same haploid strains (A to D) used for the Southern blot (Fig. 4B) were cultured in YPAD medium with shaking at 30°C for 30 h. The cell wall fractions were prepared and treated with SDS to remove noncovalently bound proteins. Cell wall proteins were solubilized with RPI, and the supernatants were analyzed by Superdex 200 gel filtration chromatography. The column was eluted isocratically with 10 mM Tris-HCl (pH 8.0) containing 150 mM NaCl and 0.05% sodium azide with a flow rate of 1 ml/min. The arrow indicates the position of Sed1p in all panels.