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. 2023 Nov 27;12(12):3656–3668. doi: 10.1021/acssynbio.3c00444

Figure 1.

Figure 1

Signal peptide screening for GLuc secretion by B. subtilis. GLuc activities in the cell and culture supernatant fractions of B. subtilis IIG-Bs27-47-24 grown for 16 h in LB medium and producing GLuc with N-terminal signal peptide (SP) fusions as indicated were measured using the Gaussia Luciferase Glow Assay. The measured data in relative light units (see Supporting Table S2) was normalized, wherein the highest measured activity in the supernatant fraction of the bacteria producing GLuc fused to SPepr+1 was set to 100%.