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. 2023 Dec 18;21:362. doi: 10.1186/s12964-023-01394-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — SHP2 is activated in db/db mice. A Levels of p-SHP2, p-ERK and their basal protein expression in kidney biopsies of db/db mice were measured by Western blot analysis. Normal kidey biopsies of WT mice were used as control samples. B, C The intensity of potein bands (A) was quntified by ImageJ and the ratio of p-SHP2/SHP2 (B) and p-ERK/ERK (C) was shown right. Student’s test, **, p < 0.01; ***, p < 0.001 (n = 6). D Immunostaining of p-SHP2 on mouse normal kidney sections (n = 6) and kidney biopsy samples of db/db mice (n = 6) was detected by IHC experiment. The integrated optical density (IOD) of p-SHP2 expression was shown right. Student’s test, ***, p < 0.001. E SHP2 immune complex PTP assay was performed using pNPP as a substrate on normal kidney samples (n = 6) from WT mice and DN kidney samples from db/db mice (n = 6). Immunoblotting control for immunoprecipitated SHP2 was shown below. Student’s test, ***, p < 0.001