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. 2023 Dec 18;21:362. doi: 10.1186/s12964-023-01394-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — PHPS1 alleviates renal injury in db/db mice. A WT and db/db mice were adminstrated i.p. with 8 mg/kg/d PHPS1 for 12 weeks, starting at 8 weeks to 20 weeks of age. Injecton of equal volume of vehicle was used as control (Ctrl). Each group included 6 mice. The levels of p-SHP2 and basal SHP2 protein were determined by Western blot analysis. B-D Mice were treated as in (A). Mouse kidneys in each group were harvested and weighted (B). The urinary albumin and creatinine levels were measured using enzyme-linked immunosorbent assay (ELISA), and the serum creatinine level (C) and urine albumin-to-creatinine ratio (UACR) (D) were presented. Student’s test, *, p < 0.05; **, p < 0.01; NS, not significant (n = 6). E Histologic examinations of renal sections from each group were performed via hematoxylin and eosin (HE) staining and periodic acid-Schiff (PAS) staining. Masson trichrome staining and IHC staining of collagen-I were employed to measure collagen fiber deposition. F The tubulointerstitial injury in (E) was semiquantified and the score in each group was depicted. Student’s test, **, p < 0.01; NS, not significant (n = 6). G The mRNA level of α-SMA in renal tissue was detected by qRT-PCR analysis. Student’s test, ***, p < 0.001; NS, not significant (n = 6)