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. 2023 Dec 5;14:1287256. doi: 10.3389/fimmu.2023.1287256

Figure 2.

Figure 2

Ectopic expression of SIRP-ß2 in hematopoietic cancer cell lines augments adhesion and their macrophage-mediated phagocytosis. (A) Expression of SIRP-ß2 on THP-1.EV and THP-1.SIRP-ß2 cells determined by flow cytometry. (B) Representative pictures of adherend THP-1.EV and THP-1.SIRP-ß2, with and without PMA treatment. (C). Quantitative real-time cell adhesion analysis of THP-1.EV and THP-1.SIRP-ß2 upon stimulation with PMA, using Xcelligence. (D) CD11b expression of THP-1.EV and THP-1.SIRPß2 after PMA stimulation (n=3). (E) Quantitative adherent cells in field of view (FOV) of THP-1.EV/HL60.EV and THP-1.SIRP-ß2/HL60.SIRP-ß2. (F) Illustrative flow cytometry data pictures of THP-1 mediated phagocytosis in co-culture with Daudi cells with RTX (1µg/ml), for 3 h (G) Representative pictures of THP-1 mediated phagocytosis incubated with Daudi cells for 3 h, treated with RTX (1 µg/ml). (H) Quantitative experimental THP-1 mediated phagocytosis of Daudi cells in combination with RTX (1 µg/ml), Inhibrx (1 µg/ml) and B6H12 (1 µg/ml) (n=4). (I) Quantitative THP-1 mediated phagocytosis of Ramos and U2932 (+ RTX, 1 µg/ml) and DLD-1 and OVCAR-3 (+ CTX, 1 µg/ml) (n=3). (J) CD11b staining on CB derived EV/SIRP-ß2 macrophage (n=3). (K) Quantitative CB-derived macrophage phagocytosis of Daudi, Ramos, U2932 and SUDHL-6, incubation 3 h. p values are indicated as: *** p < 0.001, ** p < 0.01, and * p < 0.05. ns (not significant).