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. 2023 Dec 5;14:1287256. doi: 10.3389/fimmu.2023.1287256

Figure 5.

Figure 5

Interactome analyses of SIRP-ß2 identified DAP12 as signaling component and a strong association of SIRP-ß2 with MHC-class I and potentiate HPV-E7-specific T cell responses. (A) HLA-ABC expression of THP-1.EV, THP-1.SIRP-ß2 and THP-1.SIRP-ß2.K202L determined by flow cytometry. (B) mRNA expression of HLA-A2 (CT value 30 (EV), 26 (SIRP-ß2), 27 (SIRP-ß2.K202L)). (C) HLA-B (CT value 24 (EV), 21 (SIRP-ß2), 22 (SIRP-ß2.K202L)). (D) HLA-C (CT value 25 (EV), 25 (SIRP-ß2), 26 (SIRP-ß2.K202L)) n=3. (E) RNAseq analysis from THP-1.EV, THP-1.SIRP-ß2 and THP-1.SIRP-ß2.K202L, ~300 differently regulated genes. (F, G) Major upregulation of MHC-II from RNAseq analysis. (H) Quantitative mRNA HLA-DR expression on THP-1.EV, THP-1.SIRP-ß2 and THP-1.SIRP-ß2.K202L. (I) Activation of Jurkat Nuclear Factor of Activated T-cells (NFAT)-E7 with THP-1.EV, THP-1.SIRP-ß2 and THP-1.SIRP-ß2.K202L, including different Effector to Target (E:T) ratio’s. (J) Expression of CD69 and CD25 on CD4/CD8 T cells co-cultured with THP-1.EV, THP-1.SIRP-ß2 and THP-1.SIRP-ß2.K202L and E7 peptide for 6 h (n=4). (K) Quantitative IFN-γ secretion by primary E7-TCR specific T cells in co-culture with THP-1.EV, THP-1.SIRP-ß2 and THP-1.SIRP-ß2.K202L and E7 peptide for 48 h (n=5). p values are indicated as: *** p < 0.001, ** p < 0.01, and * p < 0.05. ns (not significant).