Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 19;10:66. doi: 10.1186/s40779-023-00499-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — IFN-inducible GTPase GBP2 mediates production of large and hyperreactive platelets post IR. a Relative IFN-β mRNA expression in BM mMKs of mice at indicated dpi (n = 3). b IFN-β level in BM extracellular fluid of mice at indicated dpi was assessed by ELISA (n = 5). c Western blotting analysis and quantification of IFN-β and p-STAT1 expression in BM mMKs of mice at 1 dpi (n = 3). d IFN-β and p-STAT1 expression in BM mMKs of mice at 1 dpi were assessed by immunofluorescence staining. Scale bar = 20 μm. e Flow cytometric analysis and quantification of IFN-β and p-STAT1 expression in BM mMKs of mice at 1 dpi (n = 5). f Flow cytometric analysis and quantification of IFN-β and p-STAT1 expression in BM mMKs of Sting^fl and Sting^cko mice at 1 dpi (n = 5). g MPV in peripheral blood of Ifnar1^fl and Ifnar1^cko mice at indicated dpi (n = 5). h CD62P expression in response to thrombin on washed platelets from Ifnar1^fl and Ifnar1^cko mice at indicated dpi (n = 4–5). i Basic and relative mRNA expression of IFN-inducible GTPases as assessed by microarray in BM mMKs of mice at indicated dpi (n = 3). j Flow cytometric analysis and quantification of GBP2 expression in BM mMKs of mice at 3 dpi (n = 5). k Western blotting analysis and quantification of GBP2 expression in BM mMKs of mice at 3 dpi (n = 3). l FSC-area and CD62P expression in response to thrombin of PLPs from Meg-01 cultures with or without siGBP2 treatment at 3 dpi (n = 5). Data represent mean ± standard deviation. ^*P < 0.05, ^**P < 0.01, compared to Ctrl, IR/Sting^fl, IR/Ifnar1^fl or siCtrl as indicated. Two-tailed unpaired student’s t-test. IFN interferon, GBP2 guanylate-binding protein 2, mMK mature megakaryocyte, Ctrl control, IR ionizing radiation, BM bone marrow, dpi day post IR, BMECF BM extracellular fluid, ELISA enzyme-linked immunosorbent assay, STAT1 signal transducer and activator of transcription 1, STING stimulator of interferon genes, IFNAR1 IFN‐α/β receptor 1, MPV mean platelet volume, FSC forward scatter, PLP platelet-like particle, siGBP2 siRNA of GBP2, DAPI 4′,6-diamidino-2-phenylindole, GAPDH glyceraldehyde-3-phosphate dehydrogenase