TABLE 2.

Characteristics and laboratory results of the samples used for protocol validation patients and clinical samples and laboratory results ^a

Code	Township	Age b	Sex	Symptoms onset	Days of symptoms	RT-PCR-alpha (2015)	RT-PCR-alpha (2021)	rRT-PCR-VEE	Ct values	IgM-VEEV	IgM-MADV	IgG-VEEV	IgG-MADV	PRNT-veev c	PRNT-MADV c
258384	El Real	0–9	M	Aug. 2015	0	pos	neg	pos	29.3	neg	neg	neg	neg	<1:20	<1:20
267738	Cemaco	0–9	M	July 2017	3	neg	neg	pos	37.8	pos	neg	neg	neg	<1:20	<1:20
267411	Tucuti	0–9	F	July 2017	5	neg	neg	neg	– d	pos	neg	pos	pos	1:40	1:40
258380	El Real	0–9	F	Aug. 2015	1	pos	neg	neg	–	neg	neg	neg	neg	<1:20	<1:20
267410	Yaviza	0–9	F	July 2017	2	neg	neg	neg	–	pos	neg	pos	neg	<1:20	<1:20
258657	Yaviza	10–19	M	Sept. 2015	0	pos	neg	pos	28	neg	neg	neg	neg	<1:20	<1:20
258535	Nicanor	20–29	F	Sept. 2015	2	pos	neg	neg	–	neg	neg	neg	neg	<1:20	<1:20
258401	La Palma	20–29	M	Aug. 2015	2	pos	neg	pos	29	neg	neg	neg	neg	<1:20	<1:20
258395	Metetí	30–39	M	Aug. 2015	2	neg	neg	pos	37	pos	neg	neg	neg	<1:20	<1:20
258399	El Real	30–39	M	Aug. 2015	1	pos	neg	pos	26	neg	neg	neg	neg	<1:20	<1:20
258385	El Real	30–39	M	Aug. 2015	2	pos	neg	pos	37	neg	neg	neg	neg	<1:20	<1:20
258398	El Real	30–39	M	Aug. 2015	0	pos	neg	pos	27	neg	neg	pos	neg	<1:20	<1:20
258536	Metetí	30–39	F	Sept. 2015	2	pos	neg	neg	–	neg	neg	neg	neg	<1:20	<1:20
258386	El Real	30–39	M	Aug. 2015	5	pos	neg	pos	34	neg	neg	neg	neg	ND	ND
258379	El Real	≥40	F	Aug. 2015	2	pos	neg	pos	31	neg	neg	neg	neg	<1:20	<1:20

^a Acute samples selected from the 2015 and 2017 alphavirus outbreaks in Darien Province. Ct, cycle threshold; neg, negative; pos, positive; and ND, not done.

^b Age categories in years.

^c Base on PRNT-80.

^d -, not done and postive results and higligthed in bold.