TABLE 3.
HSV PCR detection rates according to clinical subtypes and sampling techniques in the literature a
| Reference | HSK subtypes | Type of sampling | Detection rate | Viral load | Sensitivity | Specificity |
|---|---|---|---|---|---|---|
| Fukuda et al. (19) | Epithelial: n = 27 | Tears | 100% | 6.4 × 105 (1) | NA | NA |
| Stromal: n = 14 | Tears | 57% | 1.4 × 105 (1) | |||
| Kakimaru-Hasegawa et al. (20) | Epithelial: n = 15 | Tears/scraping | 100% | 3.5 × 105/107 (1) | NA | NA |
| Stromal: n = 10 | Tears/scraping | 50% | 4.7 × 102/2.8 × 105 (1) | |||
| Endothelial: n = 8 | Aqueous humor | 25% | 2.9 × 102 (1) | |||
| Fukuda et al. (18) | Epithelial: n = 37 | Tears | 81% | 3.9 × 105 (2) | NA | NA |
| Stromal: n = 22 | Tears | 59% | 8.9 × 105 (2) | |||
| Uveitis: n = 7 | Aqueous humor | 14.30% | 3.8 × 104 (2) | |||
| Satpathy et al. (14) | Not specified | Tears | 18.30% | No quantifications | 100% (3) | 90.7% (3) |
| n = 153 | Scraping | 36.60% | 100% (3) | 71.3% (3) | ||
| Shoji et al.(22) | Epithelial: n = 23 | Tears | 78.30% | 2.3 × 104 (1) | 55.8% (4) | 100% (4) |
| Stromal: n = 9 | Tears | 33.30% | 3.1 × 102 (1) | |||
| Qiu et al. (21) | Stromal: n = 146 | Tears | 11% | NA | 11% (4) | 100% (4) |
| Present study | Epithelial: n = 39 | Tears | 97.40% | 3.3 × 105 (2) | 97.4% (4) | 100% (4) |
| Stromal without ulceration: n = 74 | Tears | 8.10% | 219 (2) | 8.1% (4) | 98.1% (4) | |
| Stromal with ulceration: n = 39 | Tears | 48.70% | 4.3 × 104 (2) | 48.7% (4) | 100% (4) | |
| Endotheliitis: n = 44 | Tears | 13.60% | 629 (2) | 13.6% (4) | 100% (4) | |
| Keratouveitis: n = 17 | Tears | 23.50% | 40 (2) | 23.5% (4) | 100% (4) | |
| Neurotrophic: n = 27 | Tears | 11.10% | 954 (2) | 11.1% (4) | 100% (4) |
a
(1) Copies/sample, (2) copies/mL, (3) compared to cell culture, (4) compared to clinical diagnosis. NA, non available.