Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 30;137(5):jcs261258. doi: 10.1242/jcs.261258

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 4. — Interacting partners of ATP7A and ATP7B. (A) Schematic showing APEX2-tagged ATP7A and ATP7B constructs. (B) Gene Ontology (GO) analysis of 892 proteins identified in the MS data with top 20 groups of biological processes are plotted according to fold enrichment; 18 of the 20 biological process groups are related to protein trafficking (written in black). (C) Venn diagram showing proteins pulled down by APEX2-tagged ATP7A and ATP7B in the proximity biotinylation assay. Trafficking regulators are sorted based on previous reports. Proteins marked in magenta are putative trafficking regulators at TGN or post-TGN compartments. The ‘*’ mark indicates multiple subunits of those proteins are detected. (D) Immuno-pulldown (IP) of mKO2–HA–ATP7A and GFP–ATP7B and probed against AP-1 using rabbit anti-AP1M1 antibody, indicating interaction of AP-1 with both the copper ATPases. WCL, whole-cell lysate (10%). Blot representative of five repeats.