Figure 7.

Immunohistochemistry of EGR1 in pulmonary arteries of patients with Down syndrome and PAH. (A) Representative images of immunohistochemistry of EGR1 in the lung tissues of healthy control (upper panels) and patients with DS and PAH (lower panels). Scale bar: 200 μm. (B) Representative images of quantitative evaluation for EGR1 positive pulmonary ECs. The parentages of 3,3′-Diaminobenzidine (DAB) positive nuclei of pulmonary arterial ECs were counted. The black arrowheads indicate EGR1-positive nuclei and the white arrowhead indicates EGR1-negative nucleus. Scale bar: 50 μm. (C) EGR1-positive cell ratio of pulmonary ECs in DS is significantly higher than that of healthy control. Seven arteries and nine arteries were analyzed in healthy control and in DS, respectively. Data are presented as mean ± SEM. Data were analyzed by an unpaired Student’s t test. ^***P < 0.001.