FIG. 1.

Restriction maps of plasmids harboring the P. putida rpoS gene. Plasmids pMIR2-9 and pMIR1-34 are two cosmids from a P. putida library carrying a gene homologous to the rpoS gene of E. coli. Plasmid pMIR61 contains the 2.5-kb BamHI/NheI fragment of pMIR11 (Table 1) inserted in the BamHI and XbaI sites of pUNØ19A (Table 1). Plasmid pMIR13450 carries the 3.4-kb EcoRI fragment of pMIR1-34 inserted in pUNØ19. Cosmid pMIR13415 harbors a mini-Tn5/′Sm element in the 3.4-kb EcoRI fragment of pMIR1-34 (Table 1). Plasmid pMIR492 is the result of inserting a luxAB cassette at position 43 inside the ORF rpoS. (The SalI/BamHI fragment from pUJ20 [Table 1], carrying the genes luxAB from Vibrio harveyi, after filling in the single-strand ends, was inserted in the unique AatII site of pMIR61 after removal of the single-strand protruding ends.) Plasmid pMIR592 was the result of inserting the fusion rpoSp::luxAB as a filled-in KpnI/SphI fragment from pMIR492 in the unique SmaI site of pKNG101 (Table 1). The plasmids listed on the right represent the relevant cloning vectors omitted from the maps. A, AatII; B, BamHI; E, EcoRI; K, KpnI; N, NheI; P, PstI; S, SphI.