FIG. 5.

Identification of the mtbA, mtmCB, and mtmCBPorf1 transcripts. Northern analyses of total RNA isolated from M. barkeri grown on MMA were performed with the same blot and with probes complementary to the regions designated in the diagram by the boxed numbers. The probes were complementary to the following positions of the contiguous nucleotide sequence: probe 1, 933 to 1010; probe 2, 1775 to 1792; probe 3, 2384 to 2403; probe 4, 3723 to 3741; probe 5, 4777 to 4794; probe 6, 6549 to 6566; and probe 7, 6750 to 6768. Molecular size standards are indicated to the left, while arrows designating the migration positions of the three transcripts are located to the right. Probes 6 and 7 gave essentially the same autoradiograms as those indicated for probe 5 and 2, respectively.