FIG. 6.

Identification of initiation sites of mtbA, mtmCB, and mtmCBPorf1 transcripts. Primers were annealed with total RNA isolated from M. barkeri grown on MMA. The products of primer extension for the mtbA (A) and mtm (B) transcripts are shown adjacent to the DNA sequencing ladders generated with the same primers. The antisense nucleotide sequence is shown to the right with the 5′ terminus and direction of transcription indicated by an arrow. The positions of the transcriptional starts are indicated in Fig. 1.