Table 3.
The HLA-A2 binding affinity and stability of CT23 candidate peptides and control peptides.
| Epitope | MFI# | FI## | DC50* |
|---|---|---|---|
| ALLTPTWKA(P1) | 15223.5 | 1.27 | 6-8 h |
| RLSNNVEEL(P2) | 13851.5 | 1.06 | 4-6 h |
| VLCSQPVSI(P3) | 12470 | 0.86 | 6-8 h |
| QLPHTEALL(P4) | 14925 | 1.22 | 6-8 h |
| MIMENIQEL(P5) | 13690.5 | 1.04 | 6-8 h |
| LLASQSLSI(P6) | 14103 | 1.10 | 4-6 h |
| ALLVLCYSI(P7) | 21424.5 | 2.19 | 6-8 h |
| KTPFVSPLL(P−) | 11116 | 0.66 | 4-6 h |
| GILGFVFTL(P+) | 15432 | 1.30 | 6-8 h |
#: MFI: mean fluorescence intensity.
##: FI = (mean FITC fluorescence with the given peptide – mean FITC fluorescence without peptide)/(mean FITC fluorescence without peptide).
*: DC50 was defined as the time (h) required for 50% dissociation of the HLA-A2/peptide complex stabilized at t = 0 h.