Figure 3.

The principle of CUT&Tag and CUT&Tag variants. In the CUT&Tag experiment, the primary antibody recognizes the target protein and then mediates the binding of the secondary antibody and the protein A/G-Tn5 fusion protein. Tn5 can precisely target and cleave the DNA sequence near the target protein in the presence of Mg²⁺. Simultaneously, the sequencing adapters will be inserted into both ends of DNA fragments by Tn5. For tissue section, spatial-CUT&Tag and MERFISH can be conducted. For cell suspension, CUT&Tag and CUT&Tag variants can be performed.