FIG. 4.

SDS-PAGE and Western blot of the proteolipid from M. mazei expressed in E. coli. ahaK was expressed by raising the temperature to 42°C at an OD₆₀₀ of 0.1. Cells were harvested at an OD₆₀₀ of 1. Protoplasts were prepared, lysed by osmotic shock, and separated into cytoplasm and membrane fractions, and the membrane fractions were subjected to SDS-PAGE (a) and Western blotting (b). Lanes 1, DK8(pJLA603), not induced; lanes 2, DK8(pJLA603), induced; lanes 3, DK8(pRT200), not induced; lanes 4, DK8(pRT200), induced.