Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 1;15(2):2288200. doi: 10.1080/19490976.2023.2288200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

PMC Copyright notice

Figure 2. — Characterization of EVs derived from four sources (G+ and G- bacterial medium, eukaryotic cell medium, and stool) by the same serial purification method (DG+UC). Samples were divided into several fractions by DG; fractions (F4-F13) were chosen for the characterization of EVs. F5 and F6, F8 and F9 were pooled, respectively. (a) Fractional distribution of particle concentration (left Y-axis, shown in a bar plot) and protein concentrations (right Y-axis, shown with a red line) from (G-)EcBEVs, (G+)BcBEVs, EEVs and stEVs. Results are shown as the means ± SDs of experiments performed in triplicate. (b) Representative TEM images of three major fractions (F5&6, F7 and F8&9) from four resources. (Scale bar = 0.2 µm) The yellow arrows indicate particles with a shape that conforms to the criteriaof EVs, and the red arrows indicate some linear structures, which may be fragments of bacterial flagella or pili. (c) Comparison of the particle sizedistribution, which has been normalized, (by converting the mode of each group to 1) of three main fractions from four different sources determined by NTA. Each line represents the average of two samples with three technical replicates.