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. 2023 Dec 3;33:483–496. doi: 10.1016/j.bioactmat.2023.11.017

Fig. 3.

Fig. 3

Evaluations of CMS performance in cellular level. a) Cell viabilities of 4T1 cells after incubation with various concentrations of CMS and CS for 24 h (n = 5). b) Cell viabilities of 4T1 cells and 3T3 cells after incubation with various concentrations of CMS for 24 h (n = 5). c) Intracellular GSH levels of 4T1 cells after treatment with various concentrations of CMS (32, 64 and 128 μg/mL, n = 3). d) CLSM images and e) flow cytometer analysis of DCFH-DA-stained 4T1 cells after treatment with different concentrations of CMS (scale bar = 30 μm). f) CLSM images of 4T1 cells stained with JC-1 after treatment with different concentrations of CMS (scale bar = 20 μm). g) Changes of intracellular ATP contents of 4T1 cells after CMS treatment (n = 3). Data represented as mean values ± SD. *, ** and *** represent statistically significant differences at P < 0.05, P < 0.01 and P < 0.001, respectively.